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What is "chromosome walking"?

A technique with which an unknown region of a
chromosome can be explored. It is generally used
to isolate a locus of interest for which no probe
is available but that is known to be linked to a
gene which has been identified and cloned. A
fragment containing a known gene is selected and
used as a probe to identify other overlapping
fragments which contain the same gene. The
nucleotide sequences of these fragments can then
be characterized. This process continues for the
length of the chromosome.

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